Pharmacological and Antioxidant Activities of Ethanol Extract of Diospyros blancoi

Lokman Hossain


The Ethanol extract of leaves of Diospyros blancoi (Ebenaceae) was tested for its pharmacological and antioxidant activities. Acetic acid induced writhing method, Brine shrimp lethality bioassay and disc diffusion method were applied for determining analgesic, cytotoxic and antibacterial activity, respectively. To investigate antioxidant activity, 2, 2-diphenyl-1-picryl-hydrazil (DPPH) free radical scavenging, total phenolic and flavonoid contents were applied. The extract produced dose-dependent analgesic activity in acetic acid-induced writhing test. Significant antibacterial and cytotoxic activity were also observed. In DPPH radical scavenging assay, the extract showed the IC50 value of 59 µg/mL (Ascorbic acid, 17 µg/mL). Total phenolic and flavonoids contents were 62.54 mg GAE and 9.63 mg QE/g of dry powder, respectively. In HPLC analysis, Catechin hydrate, Rutin hydrate, Vanillic acid, p-coumaric acid, and Quercetin were detected in the extract (39.51, 20.67, 1.68, 1.52, 1.08 mg/100 g of dry extract, respectively). Presence of polyphenolic compounds in the extract might have attributed towards the observed bio-activity.


Pharmacological activity; Antioxidant activity; HPLC; Polyphenols.

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Rates S.M.K., Review: plants as source of drugs, Toxicon 39 (2001) 603-613.

Hossain M.L., Monjur-Al-Hossain A.S.M., Sadhu S.K., HPLC Profiling and Evaluation of In-vitroAntioxidant Activity of Cirsium arvense L., (Family: Asteraceae). J Pharmacogn Phytochem, 5(2016) 272-277.

Bauer A.W., Kirby W.M.M., Sherris J.C., Turck M., Antibiotic susceptibility testing by a standardized single disk method, Am J Clin Pathol 45 (1966) 493-496.

Ahmed F., Das P.K., Islam M.A., Rahman K.M., Rahman M.M., Selim M.S.T., Antibacterial activity of Cordyline terminalis Kunth Leaves, J Med Sci, 3 (2003) 418-422.

Meyer B.N., Ferrigni N.R., Putnam J.E., Jacobsen J.B., Nicholsand D.E., Mclaughlin J.L., Brine shrimp; A convenient general bioassay for active plant constituents, Planta Medica, 45 (1982) 31-34.

Basak A., Hossain M.L., Parvin M.N.., Evaluation of phytochemical and pharmacological activities of Bacopa monnieri (L.), Int J Sci Rep 2 (2016) 242-247.

Sadhu S.K., Okuyama E., Fujimoto H., Ishibashi M., Seperation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities, Chem Pharm Bull 51 (2003) 595-598.

Wolfe K., Wu. X., Liu R.H., Antioxidant activity of apple peels, J. Agric. Food Chem. 51 (2003) 609-614.

Monic S., Yerram R.B., Jagadeesh B., Phytochemical screening and in-vitro antioxidant activity of aqueous and hydroalcoholic extract of Bacopa monnieri Linn, IJPSR, 3 (2012) 3418-3424.

Khan N., Jahan I.A., Khan T.A., Rahman M.H., Abdullah A.T.M., Rahman S.E., Hossain M.L., Hossain H., Antioxidant activities and HPLC assay of bioactive polyphenols of the ethanolic bark extract of Xylocarpus moluccensis (Lamk.) Roem, Int J Pharm Phytopharmacol Res. 3 (2014) 357-361.

Brown J.E., Rice-Evans C.A., Luteolin rich artichoke extract protects low-density lipoprotein from oxidation in-vitro, Free Radical Res, 29 (1998) 247-255.

Gil M.I., Ferreres F., Tomas-Barberan F.A., Effect of postharvest storage and processing on the antioxidant constituents (flavonoids and vitamin C) of fresh-cut spinach, J Agric Food Chem, 47 (1999) 2213-2217.

Ksouri R., Fallah H., Megdchi W., Trabelsi N., Mhamdi B., Chaieb K., Bakrouf A., Magny C., Abdelly C., Antioxidant and antimicrobial activities of edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents, Food Chem Toxicol, 47 (2009) 2083-2091.

Han D.H., Faubion J., Rooney M., Sorghum L.W., Phenolic acids, their high performance liquid chromatography separation, and their relation to fungal resistance, J Cereal Sci, 60 (2007) 255-261.

Mitchell G., Gattuso M., Grondin G., Marsault E., Bouarab K., Malouin F., Tomatidine Inhibits Replication of Staphylococcus aureus Small-Colony Variants in Cystic Fibrosis Airway Epithelial Cells, Antimicrob Agents Chemother 55 (2011) 1937-1945.

Vikram A., Jesudhasan P.R., Jayaprakasha G.K., Pillai S.D., Patil B.S., Citrus limonoids interfere with Vibrio harveyi cell-cell signalling and biofilm formation by modulating the response regulator, LuxO Microbiology 157 (2011) 99-110.

Ozçelik B., Kartal M., Orhan I., Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids and phenolic acids, Pharm Biol 49 (2011) 396-402.

Ortega R.M., Importance of functional foods in the Mediterranean diet, Public Health Nutr 9 (2006) 1136-40.

Gulcin I., Kufrevio I., Oktay M., Buyukokuro M.E., Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.), J Ethnopharmacol. 90 (2004) 205-215.

Ahmadiani A., Hosseiny J., Semnanian S., Javan M., Saeedi F., Kamalinejad M., Saremi S., Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract, J Ethnopharmacol. 72 (2000) 287-292.

Narayana K.R., Reddy M.S., Chaluvadi M.R., Bioflavanoids classification, pharmacological, biochemical effects and therapeutic potential, Indian J Pharmacol 33 (2001) 2-16.

Ismet A.J., Proity N.A., Nasima K., Tanzir A.K., Mohammad M.R., Arpona H., Hemayet H., Comparative study of anti-nociceptive activity and phenolic content of the ethanol extracts of Piper nigrum and Piper longum fruits, Int J Pharm Sci Rev Res. 27(2014) 47-52.

Kulisica T., Radonicb A., Katalinicc V., Milosa M., Use of different methods for testing antioxidative activity of oregano essential oil, Food Chem. 85 (2004) 633-640.

Hossain M.L, Monjur-Al-Hossain A.S.M., Saha S., Sadhu S.K., Assessment of Biological Activity on Cirsium arvense L., Algerian J. of Nat. Products 5 (2017) 417-426.



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