Hide
Show all
Email this article Histological study and cytotoxic effect of leaf of Globularia alypum L
Abstract
The phenomenon of drug resistance poses a major problem to a successful chemotherapy. Therefore, we have to look for newer component with effective modes by incorporating them into the test bench. Our purpose was to study the localization site of resin of Globularia alypum (L.). Also, we investigated its antitumor activity against the Hep-2 cell and Vero cell in vitro by the observation under an inverted microscopy. Hep-2 cells and Vero cell were seeded at a density of 105 or 106 cells /mL and cultured in the presence of the various concentrations of the extract for 24 h. A study of the anatomy of G. alypum using double coloration has highlighted the presence of globular trichomes which can be a secretary site of resin. An in vitro cytotoxicity experience showed that the most of Hep-2 cell and Vero cell in monolayer with a density of 106 cells/well were destroyed with concentration of 26 and 13 mg/ml.
Â
Â
References
Preedy V.R; Watson, R.R. Handbook of Disease Burdens and Quality of Life. New York: Springer 2010; p. 4161.
Hicks R. Understanding Cancer. Australia: Lulu. Huon media 2009; p. 55-56.
Candelaria M; Gallardo-Rincón; Arce C; Cetina L; Aguilar-Ponce ;J.L Arrieta O; Serrano A; Perez- Plasencia C; Gonzalez-Fierro A; de la Cruz-Hernandez E; Revilla-Vazquez A; Chavez-Blanco A; Trejo-Becerril C; Perez-Cardenas E; Taja-Chayeb L; Camargo M.F; Robles E and Dueñas-Gonzalez A. A phase II study of epigenetic therapy with hydralazine and magnesium valproate to overcome chemotherapy resistance in refractory solid tumors ,BMC Cancer 2007; 7:A27.
Watson R.R; Preedy V.R and Zibadi S. Polyphenols in Human Health and Disease. Academic Press: Elsevier 2013; p. 727.
Aїt Youssef M. Les plantes médicinales de Kabylie. Paris: Ibis Press 2006; p155-159.
Ziyya A; Legssyer A; Mekhfi H; Dassouli A; Serhrouchni M and Benjelloun W. Phytotherapy of hypertension and diabetes in oriental Morocco. J. Ethnopharmacol 1997 ; 58(1): 45-54.
Jouad H; Maghrani M and Eddouks M., 2002. Hypoglycaemic effect of Rubus fructicosis L. and Globularia alypum L. in normal and streptozotocin-induced diabetic rats. J. Ethno –pharmacol 2002; 81(3): 351–6.
Leporatti ML ; Ghedira K. Comparative analysis of medicinal plants used in traditional medicine in Italy and Tunisia, Journal of Ethnobiology and Ethnomedicine 2009; 5:31.
Es-Safi N.E; Khlifi S; Kerhoas L; Kollmann A; El Abbouyi A and Ducrot P.H. Antioxidant Constituents of the Aerial Parts of Globularia alypum growing in Morocco, J. Nat. Prod 2005; 68: 1293-1296.
Fehri B; Tebbett I; Freiburger B; Karlix J. The immunosuppressive effects of Globularia alypum extracts, Phytoth Res 1996; 10: 539–540.
Ben Mansour R; Gargouri B; Gargouri B; Elloumi N; Ben Haj Jilani I; Ghrabi-Gammar Z and Lassoued S. Investigation of antioxidant activity of alcoholic extract of Globularia alypum L. Journal of Medicinal Plants Research; 2012; 6(25):4193-4199.
Taskova R.M; Gotfredsen C.H and Jensen S.R. Chemotaxonomy of Veroniceae and its allies in the Plantaginaceae. Phytochemistry 2006; 67(3): 286–301.
DOI: http://dx.doi.org/10.5281/zenodo.3604984
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 sara IRKI
_________________________________________________________________________________________________________________________
All open access articles published in AJNP are distributed under the terms of the Creative Commons Attribution- 4.0 International License
The research works published in this journal are free to be accessed. They can be shared (copied and redistributed in any medium or format) and\or adapted (remixed, transformed, and built upon the material for any purpose, commercially and\or not commercially) under the following terms: attribution (appropriate credit must be given indicating original authors, research work name and publication name mentioning if changes were made) and without adding additional restrictions (without restricting others from doing anything the actual license permits). Authors retain the full copyright of their published research works and cannot revoke these freedoms as long as the license terms are followed.