Evaluation of the Anti-inflammatory Activity of Aqueous and Methanolic Extracts of Hibiscus sabdariffa and Camellia sinensis

Franklin Jesús Pacheco Coello, LILO FRANCO-DE JESUS

Abstract


Hibiscus sabdariffa and Camellia sinensis are attributed with various properties or health benefits, among which stands out is its anti-inflammatory capacity associated with the presence of bioactive compounds such as flavonoids. The aim of the research was to evaluate the anti-inflammatory effect of the extracts aqueous and methanolic of H. sabdariffa and C. sinensis by inhibition of the enzyme cyclooxygenase-2. Vegetal material corresponding to calyxes of H. sabdariffa and leaves of C. sinensis were used to obtain the extracts. The Folin-Ciocalteu and Marinova method was used for the determination of total phenolic compounds and flavonoids. On the other hand, anti-inflammatory activity was carried out using a commercial kit and a gallic acid standard as a control.The results showed a higher percentage of inhibition in the methanolic extracts of each vegetable material. Statistical difference was observed when buying the aqueous and methanolic of H. sabdariffa (p=0.025), aqueous and methanolic of C. sinensis (p=0.035) extracts. These findings allow us to conclude that the anti-inflammatory activity of the extracts is related to the content of phenolic compounds present in the plant material and to the extraction method.


Keywords


Hibiscus flower, Green Tea, Phenolic compounds, COX-2 inhibition

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References


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