Etude phytochimique et activité antioxydante de l’extrait aqueux de Pimpinella anisum L.

Amina Bekara, Nadia Ait Hamadouche, Khaled Kahloula, Nesrine Sadi, Abd-el-Kader Aoues

Abstract


L’étude expérimentale a été réalisée sur l’extrait aqueux lyophilisé des graines d’anis vert. La composition chimique de l’extrait a été analysé par la chromatographie liquide à haute performance et spectroscopie infra Rouge à transformée de Fourier. Les résultats de cette étude montre que l’extrait aqueux renferme une certaine quantité de polyphénols (2,38±0,01 mg équivalent d’acide gallique /g de poids sec de la plante), flavonoïdes (8,06 ± 0,08 mg équivalent de Quercétine / g de poids sec de la plante) et tannins (0,40±0,004 mg équivalent d’acide gallique / g de poids sec de la plante) avec l’absence d’autres groupes de composés bioactifs tel que les saponins et les stéroïdes. L’anéthole reste le composé majoritaire dans l’extrait aqueux obtenu à partir des graines d’anis vert. L’extrait de cette plante possède un pouvoir antioxydant moyen en comparaison avec les antioxydants de référence.


Keywords


Pimpinella anisum L., Activité antioxydante, Anéthole, HPLC, polyphénols, flavonoïdes.

References


Polese JM. La culture des plantes aromatiques, Artémis, (2006) 94 pages.

Peter KV. Handbook of herbs and spice, wood head publishing limited, (2001) 325 pages

Bardeau F. les huiles essentielles, Lanore, (2009) 330 pages.

Charles DJ Antioxidant properties spices, herbal and other sources, Springer (New York). (2013) .589 pages.

Garnier C .mes petites recettes magiques aux super-épices, Ledus, (2011) 203 pages.

Watson RR, Patel VB et Preedy VR .nuts and seeds in health and disease prevention, Elsevier, (2011) 1187 pages.

Tepe, AS, Tepe B Traditional use, biological activity potential and toxicity of Pimpinella species. Industrial Crops and Products (2015) 69, 153–166.

Hosseinzadeh H, Tafaghodi M , Abedzadeh S , et al .Effect of aqueous and ethanolic extracts of Pimpinella anisum L seeds on milk production in rats. Journal of Apiculture and Meridian studies http://dx.doi.org/10.1016/j.jams.2013.10.004.

Sofowora A .Medicinal plants and traditional medicine in Africa. Spectrum Books Ind, Ibadan, Nigeria. (1993) pp 289.

Wojdyło A, Oszmianґski J and Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry. (2007) 105: 940-949.

Djeridane A, Yous M, Nadjemi B, et al .Antioxidant activity extracts containing phenolic compounds. Food chem. (2006) 97:654-660.

Schandrel S H. Method in food analysis New York: Academic Press, (1970) 709 p.

Oyaizu L . Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucose amine. Jpn. J. Nutr. (1986) 44: 307-315.

Prieto P, Pineda M and Aguilar M, Spectrophotometric quantitative of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem, (1999). 269:337-341.

Sanchez-Moreno C, Larraurier JA, Saura-Caliscto F, A procedure to measure the antiradical efficiency of polyphenols. Sci Food Agr. (1998) 76:270.

Sharma V and Pandey D ,Protective Role of Tinospora cordifoliaagainst Lead-induced Hepatotoxicity, Toxicol Internat, (2010) ,17(1):12–17.

Shobha RI, Rajeshwari CU, Andallu B, Anti-Peroxidative and Anti Diabetic Activities of Aniseeds (Pimpinella anisum L) and Identification of Bioactive Compounds. AJPCT[1][5][2013]516-527

Ramya B.S and Ganesh P, Phytochemical Analysis and Comparative Effect of Cinnamomum zeylanicum, Piper nigrum and Pimpinella anisum with Selected Antibiotics and Its Antibacterial Activity against Enterobacteriaceae Family. International Journal of Pharmaceutical & Biological Archives 2012; 3(4):914-917.

Ghasemzadeh A and Neda Ghasemzadeh N , Flavonoids and phenolic acids: Role and biochemical activity in plants and human. Journal of Medicinal Plants Research. 5 (31): (2011). 6697- 6703.

Moussa AM, Emam AM, Diab YM, et al , Evaluation of antioxidant potential of 124 Egyptian plants with emphasis on the action of Punica granatum leaf extract on rats, International Food Research J. (2011), 18: 535-542.

Al-Daihan S, Al-Faham M, Al-shawi N, et al. Antibacterial activity and phytochemical screening of some medicinal plants commonly used in Saudi Arabia against selected pathogenic microorganisms. Journal of King Saud University – Science 25, (2013)115–120.

Wong S.P, Leong L.P and Koh J.H, Antioxidant activities of aqueous extract of selected plants. Food Chemistry 99, (2006)775-783.

Christova-Bagdassarian V L, Bagdassarian K S and Atanassova M S, Phenolic compounds and antioxidant capacity in Bulgarian plans (dry seeds). International Journal of Advanced Research 1(9): (2013)186-197.

Dorman D H J and Hiltunen R , Antioxidant and pro-oxidant in vitro evaluation of water-soluble food-related botanical extracts. Food Chemistry 129 (2011) 1612–1618.

Gulçın I , Oktay M, Kıreçcı E, et al Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisumL.) seed extracts. Food Chemistry 83 (2003) 371–382.

Siddhuraju P.et Becker K, the antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) Seed extracts. Food Chemistry. 101(1), (2007) 10-19.

Lu Y and Foo LY, Salvianolic acid L a potent phenolic antioxidant from Salvia officinalis. Tetrahedron letters, (2001). 42: 8223-8225.

Yang L, Gou Y, Zhao T, et al , Antioxidant capacity of extract from Calyx fruits of Roselle (Hibiscus sabdariffa L.). Afr .J. Biotechnol, (2012) 11(17), 4063-4068.

Swamy LP, Prasad VG, Krishna VB, et al, Total antioxidant potential of selected indigenous plants and culinary species. Int.J.Res.Pharm.Sci, (2014),5(1), 64-69.

Ebrahimzadeh MA, Nabavi SM, Nabavi SF, et al , Antioxidant and Antihaemolytic Activities of the Leaves of Kefe cumin (Laser trilobum L) Umbelliferae, Tropical J of Pharmac. Res. 9 (5): (2010) 441-449.

Ghasemi K, Ghasemi Y, Ebrahimzadeh MA Antioxidant activity, phenol and flavonoid contents of 13 Citrus species peels and tissues. Pak J Pharm Sci, (2009) 22(3): 277- 281.

Nabavi SM, Ebrahimzadeh MA, Nabavi SF, et al, In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharmacognosy Magazine, 4(18): (2009) 123-127.

Huda S. A. A. Mohamed, Warda S. Abdelgadir, Aisha Z. I. Almagboul.(2015). In vitro antimicrobial activity of Anise seed (Pimpinella anisum L.) International Journal of Advanced Research (2015), Volume 3, Issue 1, 359-367.

Boskabady M$, Ramazani-Assari M, Relaxant effect of Pimpinella anisum on isolated guinea pig tracheal chains and its possible mechanism(s). Journal of Ethnopharmacology 74, (2001) 83–88.

Albuquerque AA, Sorenson AL, Leal-Cardoso JH. Effects of essential oil of Corton zehntneri and of anethole and estragol on skeletal muscles. Journal of Ethnopharmacology 49, (1995) 41–49.

Mohammed M.J, Isolation and identification of anethole from Pimpinella anisum L. fruit oil. An antimicrobial study. Journal of Pharmacy Research 2009, 2(5), 915-919.

Please cite this Article as:

Amina Bekara, Nadia Ait Hamadouche, Khaled Kahloula, Nesrine Sadi et Abd-el-Kader Aoues. Etude phytochimique et activité antioxydante de l’extrait aqueux de Pimpinella anisum L., Algerian J. Nat. Products, 4:3 (2016) 299-307.

www.univ-bejaia.dz/ajnp

Online ISSN: 2353-0391

Editor in chief: Prof. Kamel BELHAMEL


Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 ALGERIAN JOURNAL OF NATURAL PRODUCTS

_________________________________________________________________________________________________________________________

 All open access articles published in AJNP are distributed under the terms of the  Creative Commons Attribution-  4.0 International License

The research works published in this journal are free to be accessed. They can be shared (copied and redistributed in any medium or format) and\or adapted (remixed, transformed, and built upon the material for any purpose, commercially and\or not commercially) under the following terms: attribution (appropriate credit must be given indicating original authors, research work name and publication name mentioning if changes were made) and without adding additional restrictions (without restricting others from doing anything the actual license permits). Authors retain the full copyright of their published research works and cannot revoke these freedoms as long as the license terms are followed.

 

 Flag Counter