Extracellular production and characterization of red pigment from Penicillium purpurogenum BKS9

Bijay Kumar Sethi, Prabhatee Parida, Santi L. Sahoo, BIKASH CHANDRA BEHERA


Fifteen fungal species were isolated from waste soil and used for the production of red pigment. Among the fifteen fungal isolates only Penicillium sp. BKS 9 displayed excellent production ability of red pigment in sabouraud’s dextrose agar medium as well as broth medium. After 28S rRNA sequencing the fungus Penicillium sp. BKS9 was identified as Penicillium purpurogenum and submitted to NCBI (Gene Bank) with Gene Bank accession number KT222270. Further, optimization of incubation period and pH was performed and it was concluded that an incubation for 18 days with pH 6.0 at 30°C are the most favorable conditions for biomass (0.877 g/50 ml) and red pigment (0.79 Abs/ml) production. The pigment used for the study was soluble in all the organic solvents taken. The effect of red pigment on germination of fifty numbers of viable and healthy seeds of Cicer arietinum was studied and confirmed that the pigment has no significant negative effect on the germination of the seeds.


Optimization; Incubation; pigment; solvents

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Kim SW, Hwang HJ, Xu CP, Na YS, Song SK, Yun JW., Influence of nutrition conditions on the mycelial growth and exopolysaccharide production in Paecilomyces sinclairii, Lett. Appl. Microbiol. 34 (2002) 389-393.

Joshi VK., Attri D., Bala A., Bhushan S., Microbial Pigments, Ind. J. Biotech. 2 (2003) 362-367.

Nagpal N., Munjal N., Chatterjee S., Microbial Pigments with Health Benefits - A Mini Review, Trends Biosci. 4: (2011)157-160.

Aberoumand A., (2011) A review article on edible pigments properties and sources as natural biocolorants in foodstuff and food Industry, World. J. Dairy Food Sci. 6:1(2011)71-78.

Ahmad WA., Ahmad WYW., Zakaria ZA., Yusof NZ., Application of bacterial pigments as Colorant. Springer Briefs in Molecular Science, (2012) pp 57-74.

Mendentsev AG., Arinbasarova AY., Akimenko VK., Biosynthesis of naphthoquinone pigment by fungi of the genus Fusarium, Appl. Biochem. Microbiol. 41: 5 (2005) 573-577.

Jittra K., Kulvadee D., Nattawut B., Tanapong B., Pranee R., Samran P., Palangpon K., Chawanee T., Dihydronaphthalenones from endophytic fungus Fusarium sp. BCC14842, Tetrahed. 67 (2011) 7540–7547.

Moita C., Feio SS., Nunes L., Joa M., Curto M., Optimization of physical factors on the production of active metabolites by Bacillus subtilis 355 against wood surface contaminant fungi, Int. Biodet. Biodegrad. 55 (2005) 261–269.

Vahidi H., Kobarfard F., Namjoyan F., Effect of cultivation conditions on growth and antifungal activity of Mycena leptocephala, Afr. J. Biotechnol. 3 (2004) 606-609.

Sameer AM., Anne SM., Ulf T., Colorimetric Characterization for Comparative Analysis of Fungal Pigments and Natural Food Colorants, J. Agric. Food Chem. 54 (2006) 7027-7035.

Hajjaj H., Klaebe A., Loret MO., Tzedakis T., Goma G., Blanc JP., Production and identification of N-Glucosylrubropuntamine and N-Glucosylmonascorubramine from Monascus ruber and occurance of electron donor-acceptor complexes in these red pigments, Appl. Environ. Microbiol. 63 (1997) 2671-2678.

Sohail M., Naseeb S., Sherwani SK., Khan S., Distribution of hydrolytic enzymes among native fungi: Aspergillus the pre-dominant genus of hydrolase producer., Pak. J. Bot. 41:5 (2009) 2567-2582.

Alexopoulos C.J., Mims CW., Introductory Mycology, 3rd eds, John Wiley & Sons, (1979) pp. 1-613.

Watanabe T., Pictorial atlas of soil and seed fungi - morphologies of cultured fungi and key to species. 2nd ed. Boca Ratón: CRC Press; 2002.

Möller EM., Bahnweg G., San dermann H., Geiger HH., A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucl Acids Res. 22 (1992) 6115 - 6116.

Saitou N., Nei M.,The Neighbor-joining method: A new method for reconstructing phylogenetic trees, Mol. Biol. Evol. 4:4 (1987) 406-425.

Felsenstein J., Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 39 (1985)783–791.

Kimura M., A simple method for estimating evolution-ary rates of base substitutions through comparative studies of nucleotide sequences, J. Mol. Evol.16 (1980) 111–120.

Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol. 28 (2011) 2731–2739.

Sethi BK., Singh S., Nanda PK., Sahoo SL., Extracellular biosynthesis of amylase by Aspergillus terreus NCFT 4269.10 using agro-residues. J. Micrbiol. Biotechnol. Res. 4 (2014) 8-14.

Said FM., Chisti Y., Brooks J., The effects of forced aeration and initial moisture level on red pigment and biomass production by Monascus ruber in packed bed solid state fermentation, Int. J. Environ. Sci. Develop. 1:1 (2010) 1-4.

Velmurugan P., Kamala-Kannan S., Balachandar V., Lakshmana- perumalsamy P., Chae JC., Oh BT., Natural pigment extraction from five filamentous fungi for industrial applications and dyeing of leather, Carbohydr. Polym. 79 (2010) 262–268.

Velmurugan P., Hur H., Balachandar V., Monascus pigment production by solid-state fermentation with corn cob substrate, J. Biosci. Bioeng. 112 (2011) 590-594.

Petit P., Lucas EMF., Breu LMA., Flenning LHP., Takahashi JA., Novel antimicrobial secondary metabolites from a Penicillium sp. isolated from Brazilian cerrado soil, Elect. J. Biotechnol. 12 (2009) 1–9.

Dhale MA., Vijay-Raj AS., Pigment and amylase production in Penicillium sp NIOM-02 and its radical scavenging activity, Int. J. Food Sci. Technol. 44:12 (2009) 2424-2430.

Jiang Y., Li HB., Chen F., Hyde K.D., Production potential of water-soluble Monascus red pigment by a newly isolated Penicillium sp., J. Agric. Technol., 1: 1 (2005)113-126.

Mapari SAS., Hansen ME., Meyer AS., Thrane U., Computerized screening for novel producers of Monascus-like food pigments in Penicillium species. J. Agric. Food. Chem. 56 (2008) 9981-9989.

Mabrouk AM., El-khrisy EAM., Youssef YA., Asem AM., Production of textile reddish brown dyes by fungi, Mal. J. Microbiol. 7:1 (2011) 33-40.

Marova I., Carnecka M., Halienova A., Certik M., Dvorakova T., Haronikova A., Use of several waste substrates for carotenoid-rich yeast biomass production, J. Env. Manag. 95 (2011) 338-342.

Mukherjee G., Singh SK., Purification and characterization of a new red pigment from Monascus purpureus in submerged fermentation, Proc. Biochem. 46 (2011)188-192,

Cho YJ., Park JP., Hwang HJ., Kim SW., Choi JWM., Yun JW., Production of red pigment by submerged culture of Paecilomyces sinclairii, Lett. Appl. Microbiol. 35 (2002) 195-202.

Chen MH., Johns MR., Effect of pH and nitrogen source on pigment production by Monascus purpureus, Appl. Microbiol. Biotechnol. 40 (1993)132-138.

Méndez A., Pérez C., Montañéz JC., Martínez G., Aguilar CN., Red pigment production by Penicillium purpurogenum GH2 is influenced by pH and temperature, J. Zhejiang Univ. Sci. B 12:12 (2011) 961-968.

Babitha S., Soccol CR., Pandey A., Effect of stress on growth, pigment production and morphology of Monascus sp in solid cultures, J. Basic Microbiol. 47:2 (2007) 118-26.

Lin TF., Demain AL., Effect of nutrition of Monascus sp. on formation of red pigments, Appl. Microbiol. Biotechnol. 36:1 1991.70-75.

Hailei W., Zhifang R., Ping L., Yanchang G., Guosheng L., Jianming Y., Improvement of the production of a red pigment in Penicilliumsp HSD07B synthesized during co-culture with Candida tropicalis, Biores. Technol. 102 (2011) 6082-6087.

DOI: http://dx.doi.org/10.5281/zenodo.262120


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