Teratogenic Effects of Ethanol Leaf Extract of Dryopteris filix –mas (L.) Schott

Earnest Oghenesuvwe Erhirhie

Abstract


This study evaluated the teratogenic profile of ethanol leaf extract of Dryopteris filix-mas. Thirty-two (32) female virgin albino mice were grouped into control and treatment groups (250, 500 and 1000 mg/kg of extract). Oestrus female mice were mated with males and pregnant female mice were administered the extract from the 6th until the 15th day of pregnancy. Morphological parameters as well as histopathologies of the femur, liver, kidneys, lungs and heart of pups in treatment and control groups were carried out. Administration of 250, 500, 1000 mg/kg of the leaf extract did not cause significant alteration (p>0.05) in morphological parameters in pups when compared with control group. There was no alteration in histo-architecture of the lungs, femurs, liver, heart and kidneys but there was poor mineralization in metaphysis of pups femur in high dose of the extract when compared to control group.


Keywords


Dryopteris filix-mas, Teratogenic profile, poor mineralization in femur, mice.

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References


Biljana BP., Historical review of plant usage, Pharmacognosy Reviews, 6: 11 (2012) 1–5.

Narayanaswamy T., Thirunavukkarasu T., Prabakar S., Ernest D., A review on some poisonous plants and their medicinal values, Journal of Acute Disease, 3: 2(2014) 85-89.

Lozano R., Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study, Lancet, 380(2012): 2095-2128.

Lisha JJ., Nisha S., Herbal medicines use during pregnancy: A review from the Middle East, Oman Medical Journal, 30:4(2015): 229–236.

Tamuno I., Omole-Ohonsi A., Fadare J., Use of Herbal Medicine Among Pregnant Women Attending A Tertiary Hospital In Northern Nigeria, Internet Journal of Gynecology and Obstetrics, 15:2 (2010) 1-8.

Chukwuma BD., Kenechi AU., Nnebue CC., Ikechukwu IM., Kevin CD., Chuka CA., Anthony CI., Irene AM., Socio-demographic determinants of herbal medicines use in pregnancy among Nigerian women attending clinics in a tertiary hospital in Imo State, South-East, Nigeria”. American Journal of Medicine Studies. 4:1 (2016) 1-10.

Ray G., Niall S., Mark JR., The History and Implications of Testing Thalidomide on Animals, The Journal of Philosophy, Science and Law,11 (2011) 1-32.

Saganuwan SA., Toxicity study of drugs and chemicals in animals: An overview, Bulgarian Journal of Veterinary Medicine. 2016 online first. 2016, DOI: 10.15547/bjvm.983.

Saleem U., Ahmad B., Hussain K., Bukhari NI, Anjum AA, Teratogenic and embryotoxic effects of latex and leaves of methanol extract of Euphorbia helioscopia L. in mice and chicken, The Journal of plant and animal sciences, 24: 2 (2014) 450-454.

Sekendar MA., Kawsarul M., Obayed MR., Khalilur MR., Aslam MH., Shah MA., Antioxidant and Cytotoxic activities of Methanol extract of Dryopteris filix-mas (L.) Schott Leaves, International Journal of Drug Development and Research, 4:2 (2012) 223-229.

Uwumarongie HO., Enike MA., Bafor EE., Pharmacognostic evaluation and gastrointestinal activity of D.filix-mas, Ewemen Journal of herbal chemistry and pharmacology research, 2:1 (2016) 19-25.

Tagarelli G., Tagarelli A., Piro A., Folk medicine used to heal malaria in Calabria (southern Italy), Journal of Ethnobiology and Ethnomedicine, 6:27 (2010) 1-16.

Beyrouthy EM., Arnold N., Delelis-Dusollier A., Dupont F., Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon, Journal of Ethnopharmacology, 120:3 (2008) 315–334.

Mandal A., Kumar-Mondal A., Studies on antimicrobial activities of some selected ferns and lycophytes in Eastern India with special emphasis on ethno-medicinal uses, African Journal of Plant Science, 5:7 (2011) 412-420.

Hamiduddin AM., Sofi G., Wadud A., Use of traditional drugs in pregnant and nursing mothers, A review of associated adverse drug reactions, Journal of Pharmaceutical Scientific Innovation, 5:1 (2016) 2277-4572.

Anosike CA., Obidoa O., Anti-inflammatory and Anti-ulcerogenic effect of ethanol extract of Coconut (Cocos Nucifera) on experimental rats, African Journal of Food, Agriculture, Nutrition and Development, 10:10 (2010) 4286-4300.

Yadav RNS., Agarwala M., Phytochemical analysis of some medicinal plants, Journal of Phytology, 3:12 (2011) 10-14.

Randhawa MA., Calculation of LD50 value from the method of Miller and Tainter of Ayub Medical College A bbottabad, Journal of Ayub Medical college Abbottabad, 21:3 (2009) 1-3.

Golalipour MJ., Ghafari S., Maleki A., Kiani M., Asadi E., Farsi M., Study of Embryotoxicity of Mentha piperita L. During Organogenesis in Balb/c Mice, International. Journal of Morphology, 29: 3 (2011) 862-867.

Behringer R, Gertsenstein M, Nagy KV, Nagy A. Selecting female mice in estrus and cheking plugs, Cold Spring Harb Protoc, (2016) 729-731.

Bancroft JD., Gamble M., Theory and Practice of Histological Techniques. Edition Benkroft. Churchill Livingstone, Edinburgh. (2002) 16-64.

Ahmed M., Acute Toxicity (Lethal Dose 50 Calculation) of Herbal Drug Somina in Rats and Mice, Pharmacology and Pharmacy, 6 (2015) 185-189.

Kovacs CS., Bone development in the fetus and neonate: role of the calciotropic hormones. Current Osteoporosis Report, 9:4 (2011) 274-283.

Hassan SW., Ladan MJ., Dogondaji RA., Umar RA., Bilbis LS., Hassan LG., Ebbo AA., Matazu IK., Phytochemical and Toxicological studies of aqueous leaves extracts of Erythrophyleum africanum, Pakistan Journal of Biological Science, 10: 21 (2007) 3815-3821.

Collins-Burow BM., Antoon JW., Frigo DE., Elliott S., Weldon CB., Boue SM., Beckman BS., Curie TJ., Alam J., McLachlan JA., Burow ME., Antiestrogenic Activity of Flavonoid Phytochemicals Mediated via the c-Jun N-terminal Protein Kinase Pathway: Cell-type specific regulation of estrogen receptor alpha, Journal of Steroid Biochemistry and Molecular Biology, 132 (2012) 186–193.

Nurcahyani N., Wirasti Y., Jamsari F., Tjong DH., Kanedi M. Methanol plant extract of Rumput Teki (Cyperus rotundus L.) causing fetal skeletal retardation in mice, European Journal of Biomedical and Pharmaceutical Sciences, 4:6 (2017) 128-131.




DOI: http://dx.doi.org/10.5281/zenodo.1336888

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