In vitro Screening of Potential Antioxidative and Toxicity of the Stembark Extracts from Sterculia setigera Del.

Aminu Muhammad, Musa Maikudi Idris, Adamu Mamman


The current investigation deals with the in vitro antioxidative and toxicity effects of methanol and chloroform extracts from the stem bark of Sterculia setigera. The stem bark of the plant was extracted using organic solvents (methanol and chloroform) to afford methanol extract (ME) and chloroform extract (CE). The extracts were evaluated for antioxidative potentials using 2.2-diphenyl-1-picrylhydraxyl (DPPH) radical, also toxicity effect of the methanol extract was investigated against Artemia salina larvae. The antioxidative effects for the extracts uncovered promising activity on the DPPH with significant scavenging effects of 80.64% and 18.3% at 1000 and 7.813 µg/mL respectively. These values were found to be comparable with those of ascorbic acid (97.80 and 49.62%) and butylated hydroxytoluene (94.44 and 20.98%). The methanol extract was found to be as toxic as the positive control (potassium dichromate) with LC50 values of 1.64 µg/mL and 1.42 µg/mL, respectively. The antioxidative and cytotoxicity properties of methanol extract from S. setigera supported the ethno-medicinal claims on the plant as a curative agent of different diseases of clinical concern.


Sterculia setigera; Cytotoxicity; Antioxidant.

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Rojas, Bustamante; Bauer. Antimicrobial activity of selected Peruvian medicinal plants. Journal of Ethnopharmacol 88 (2003) 199-204.

Refaz Ahmad Dar; Mohd Shahnawaz; Parvaiz Hassan Qazi. General overview of medicinal plants: A review. Journal of Phytopharmacology 6(6) (2017) 349-351.

Sigh R. Medicinal Plants: A Review. Journal of Plant Sciences 3(1-1) (2015) 50-55.

Tor-Anyiin; Akpuaka, M.U.; Oluma, H.O.A. Phytochemical and antimicrobial studies on stem bark extract of Sterculia setigera Del. African Journal of Biotechnology 10(53) (2011) 11011-11015.

Nahdal M. M. Taha; Barakat M. Mudawi. Antioxidant and cytotoxic activities of

Sterculia setigera Del. American journal of Research Communication 6(5) (2018) 41-49.

Partha G.; Rahaman Chowdhury Habibur. Pharmacognostic, Phytochemical and Antioxidant Studies of Adenanthera pavonina L. International Journal of Pharmacognosy and Phytochemical Research 7(1) (2015) 30-37.

Singh U.; Jialal I. Oxidative stress and atherosclerosis. Journal of Pathophysiology 13(3) (2006) 129-142.

Sas K,; Robotka H,; Toldi J,; Vécsei L. Mitochondrial, metabolic disturbances, oxidative stress and kynurenine system, with focus on neurodegenerative disorders. Journal of Neurol. Sci. 257(1-2) (2007) 221-239.

Alam Nur Md.; Nusrat Jahan Bristi; Md. Rafiquzzaman. Review on in vivo and in

vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal 21 (2013)


Rohit Kumar Bargah; Preliminary test of phytochemical screening of crude ethanolic and aqueous extract of moringa pterygosperma Gaertn. Journal of Pharmacognosy and Phytochemistry 4(1) (2015) 07-09.

Shovon Lal Sarkar; Prianka Saha; Nigarin Sultana. In vitro evaluation of phytochemical components and antimicrobial activity of the methanolic extract ofTridax procumbens L. against pathogenic microorganisms. Journal of Pharmacognosy and Phytochemistry 5(5) (2016) 42-46.

Sharma, Om P. and Bhat, Tej K. DPPH antioxidant assay. Journal of food chemistry 133 (2009) 1202-1205.

Apurbo Kumer Saha; Md. Rashidur Rahman; Masum Shahriar; Sudip Kumar Saha; Nadezda Al Azad; Susmita Das. Screening of six Ayurvedic Medicinal Plant Extracts for Antioxidant and Cytotoxic Activity. Journal of Pharmacognosy and Phytochemistry 2(2) (2013) 181-188.

Lilybeth F. Olowa; Olga M. Nuneza. Brine shrimp lethality assay of the ethanolic extract of three selected specie of medicinal plant from Iligan City, Philippines. International Research Journal of Biological Sciences 2(11) (2013) 74-77.

McLaughlin Jerry L.; Lingling L.; Rogers; Jon E. Anderson. The use of biological assays to evaluate botanicals. Drµg Information. Journal 32 (1998) 513-524.

Otang Wilfred M.; Donald S.; Grierson; Roland N. Ndip. Assessment of potential toxicity of three South African medicinal plants using the brineshrimp (Artemia salina) assay. African Journal of Pharmacy and Pharmacology, 7(20) (2013) 1272-1279.

Asaduzzaman Md.; Dr. Md. Sohel Rana; S. M. Raqibul Hasan; Md. Monir Hossain; Nittananda Das. Cytotoxic (Brine shrimp lethality bioassay) and antioxidant investigation of Barringtonia acutangµLa (L.) International Journal of Pharma Sciences and Research 6(8) (2015) 1179-1185.

Jeremiah Oshiomame Unuofin, Gloria Aderonke Otunola and Anthony Jide Afolayen;Toxicity Evaluation of vernonia mespilipholia Less (A South African Medicinal Plant) Using Brine Shrimp. Journal of Pharmacology and Toxicology, 12(2) (2017) 103-110.

Liu J, Zhang C, Wang C and Wang Z; The antioxidant and free radical scavenging activities of extract fractions from corn silk (Zea mays L.) and related flavone glycoside. Food chem,126(1) (2011) 261-269.

Meyer B.N; N.R. Ferrigni; J.E. Putnam; L.B. Jacobsen; D.E. Nichols; J.L. McLaughlin. Brine shrimp: A convenient general bioassay for active plant constituents. Journal of Medicinal plant research 45 (1982) 31-34.

Bastos; Lima; Conserva; Andrade; Rocha; Lemos. Studies on the antimicrobial activity and Brine shrimp toxicity of Zeyheria tubercµLosa (vell). Bur. (Bignoniaceae) extracts and their main constituents. Ann. Clinical Microbiol. Antimicrobial 8 (2009) 10.1186/1476-0711-8-16.

Artanti, N.; T. Firmansyah; A. Darmawan. Bioactivities evaluation of Indonesian mistletoes (Dendrophthoe pentandra (L.) Miq.) Leaves extracts. Journal of Applied Pharmaceutical Sciences 2 (2012) 24-27.



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